Fig. 5

Complementation of the ys53 mutant by OsGSAM gene. a Identification of transgenic lines by PCR. M, DL-2000 Marker; CK, pC2300-OsGSA plasmid (PCR positive control); ys53, ys53 mutant (PCR negative control); 1–13, positive transgenic plants. b Phenotypes of ys53 mutant, the wild type (WT), and the positive transgenic plants (TPs) at seedling stage. c Phenotypes of ys53, WT, and TPs at grain filling stage. d Photosynthetic pigment contents (in mg g fresh weight^− 1) of ys53, WT, and TPs in leaves of four-week-old seedlings. e Photosynthetic pigment contents (in mg g fresh weight^− 1) in leaves of ys53, WT, and TPs at booting stage. f and g ALA-synthesising activity of rice seedlings, and total content of GSA and ALA in rice leaves at seedling stage, respectively, which were detected according to the methods as Fig. 4. Data are mean ± SD (n = 3). Error bars represent SDs of three independent experiments. * indicate statistically significant differences compared with WT at P < 0.05