Fig. 2

Map-based cloning of the ASL4 locus. a The ASL4 locus was initially mapped to a 1.03-Mb region between markers C3–16 and K5 on the short arm of chromosome 3. b ASL4 was fine-mapped to a 50-kb region between markers K40 and K29 using 1137 F₂ mutant seedlings. c Three ORFs were predicted in the region. d Struct. of ASL4. ATG and TGA indicate start and stop codons. Blue boxes indicate exons and the lines between them indicate introns. White boxes represent the 5′ and 3′ UTR. The location of the 2855-bp deletion in the asl4 allele is indicated. PCR identification of genomic DNAs (e) and cDNA (f) between wild type and asl4 mutant using primer pairs indicated in d. The actin gene was amplified as the control