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Fig. 6 | Rice

Fig. 6

From: OsGATA16, a GATA Transcription Factor, Confers Cold Tolerance by Repressing OsWRKY45–1 at the Seedling Stage in Rice

Fig. 6

OsGATA16 interaction with the OsWRKY45–1 promoter in rice. a Schematic representation of reporter plasmids for Dual-luciferase reporter analysis, with REN luciferase as an internal control (b-e) Relative LUC activity in wild-type and OsGATA16-overexpression (OE) lines with (b) OsWRKY45–1, (c) OsSRFP1, (d) OsCYL4, and (e) OsMYB30 reporter constructs. Data represent the mean ± SE from three replicates. (f) Schematic representation of recombinant plasmids for yeast one-hybrid analysis of the OsWRKY45–1 promoter. (g) Yeast one-hybrid assay results. Blue coloration is indicative of protein–promoter interaction

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