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Fig. 4 | Rice

Fig. 4

From: Two VQ Proteins are Substrates of the OsMPKK6-OsMPK4 Cascade in Rice Defense Against Bacterial Blight

Fig. 4

OsMPK4 phosphorylated OsVQ14 and OsVQ32. a and b Mutagenesis of putative MAPK phosphorylation sites of OsVQ14 (a) and OsVQ32 (b). c and d Phosphorylation assays of wild-type (WT) and mutated OsVQ14 (c) and OsVQ32 (d) by OsMPK4 in vitro. TF-His, trigger factor (TF) and histidine (His) tag. Auto., Autoradiograph; CBB, Coomassie brilliant blue staining. e In vitro kinase activity toward OsVQ14 of OsMPK4 and its constitutively active (CA) mutant. f Phosphorylation assays of wild-type (WT) and mutated OsVQ14 and OsVQ32 by OsMPK4CA in vivo. IP, immunoprecipitated

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