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Rice
Fig. 3 | Rice

Fig. 3

From: Two VQ Proteins are Substrates of the OsMPKK6-OsMPK4 Cascade in Rice Defense Against Bacterial Blight

Fig. 3

OsVQ14 and OsVQ32 interacted with OsMPK4. a and b Schematic representation of OsVQ14 truncations (a) and OsVQ32 truncations (b). Rectangles “I” and “VQ” mean domain I and VQ domain, respectively. c Interaction of OsMPK4 with OsVQ14 or OsVQ32 was analyzed by yeast two-hybrid assay. The interactions were assessed by growing yeast cells on synthetic defined premixes (SD) medium lacking (−) leucine (L), tryptophan (W), histidine (H), and adenine (A). Co-transformation of BD-53 and AD-RecT was used as positive control while co-transformation of BD-Lam and AD-RecT was used as negative control. AD, Activation domain; BD, DNA-binding domain. d Interaction of OsMPK4 with OsVQ14 or OsVQ32 in rice plants was analyzed by co-immunoprecipitation assay. Total protein was extracted from rice leaves and anti-OsMPK4 antibody was used for the immunoprecipitation (IP)

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