Fig. 6
From: Determinant Factors and Regulatory Systems for Anthocyanin Biosynthesis in Rice Apiculi and Stigmas
Both OsC1 and OsPa or OsPs were necessary for the transcriptional expression of OsDFR promoter. a Schematic representation of the effector and reporter constructs. Full-length coding regions of OsC1, OsPa and OsPs under control of the ubiquitin promoter were used as the effectors. Firefly luciferase gene LUC driven by the OsDFR promoters and the Renilla luciferase gene Ren driven by the 35S promoter were used as the reporter and internal control, respectively. b Transient dual-luciferase assays were performed in Nicotiana benthamiana leaves to investigate the effects of OsC1, OsPa and OsPs on the transcriptional expression of OsDFR. Relative LUC activity was measured by the ratio Firefly luciferase (LUC): Renilla luciferase (REN), and data are presented as means ± SD (n = 3)