Fig. 5
From: Determinant Factors and Regulatory Systems for Anthocyanin Biosynthesis in Rice Apiculi and Stigmas
OsC1 physically interacted with OsPa and OsPs. a Validation of interactions of OsC1 with OsPa and OsPs in yeast two-hybrid assays after 3 days. Transformed cells were spotted on the control (DDO, SD/−Leu/−Trp) and selective (QDO, SD/−Leu/−Trp/−His/−Ade) media. b LCI assays certified that OsC1 interacted with OsPa or OsPs. c BiFC assays for the determining interaction of OsC1 with OsPa or OsPs. The full-length OsC1 protein was fused with C-terminal YFP (Yc). Full-length OsPa and OsPs proteins were fused with N-terminal YFP (Yn), respectively