Fig. 6

Expression analysis of genes in carotenoid biosynthesis and validation of genes identified in RNA-seq analysis. qRT-PCR analysis of genes related to carotenoid biosynthesis, chlorophyll biosynthesis and validation of genes identified as DEGs in oslil3 vs WT this transcriptome analysis. Two reference genes were used as internal controls. The expression levels of genes in WT plants were set to 1. OsAtpB, Os10g21266; OsPSY1, Os06g51290; OsPSY2, Os12g43130; OsPSY3, Os09g38320; OsGGR, Os02g51080; OsPORA, Os04g58200; OsPORB, Os10g35370; OsNECD, Os12g24800; OsLUT1, Os10g39930; OsCPOX, Os04g52130; OsCHLI, Os03g36540; OsOR, Os02g43500; OsCLPC1, Os04g32560; OsCLPS1, Os08g33540; OsLHCA2, Os09g26810