Fig. 4
Genetic analysis between OsPRR37 and Ghd7. a Allele information of ghd7 mutant generated by clustered regularly interspaced short palindromic repeats (CRISPR) strategy. Black bars represent the exons, and solid line before, between and after the black bars represents the 5′ un-transcribed region, intron and 3′ un-transcribed region, respectively. Letters in the blue box is the protospacer adjacent motif (PAM) sequence. Blue letters indicate the targets sequence. Red letters indicate the mutation details. b-c Phenotype of whole plants (b) and main panicles (c) of ZH11, osprr37, ghd7 and double mutants osprr37 ghd7 (#1 and #2) grown under NLD conditions. Scale bar, 25 cm in b and 5 cm in c. d-e Heading date and yield per plant of ZH11, osprr37, ghd7, and the double mutants osprr37 ghd7 (#1 and #2) grown under NLD conditions. Data represent mean ± SD, n = 10. Different letters indicate significant differences, Duncan’s test. f-h Log10-transformed expression levels of Ehd1 (f), Hd3a (g) and RFT1 (h) in ZH11, osprr37, ghd7 and the double mutant osprr37 ghd7. Expression of indicated genes in leaves of 40-d-old plants under controlled LD conditions were determined 2.5 h before and after dawn by qRT-PCR and shown as mean ± SD of three replicates. Rice ubiquitin gene (Os02g0161900) was used for normalization. Different upper- and lower-case letters indicate significant differences at P < 0.05 by using Duncan’s test to compare the expression levels of indicated genes before and after dawn, respectively