Fig. 2
Map-based cloning of LHD7. a Chi-square test of heading-date distribution of an F2 population derived from the cross between lhd7 mutant and ZH11. b Distribution of Δ index of single nucleotide polymorphism (SNP) across 12 chromosomes. Δ (SNP index) means the absolute value of the difference of SNP index between the bulked pool and ZH11. c Genotyping examples with SNP markers used in kompetitive allele specific PCR (KASP) assay of the F2 population. Dots in different colors and grouped together represent different genotype of the SNPs as indicated. d Progeny tests of 5 recombinants. Numbers of individual plant and information of SNP markers were listed on the left and above sides, respectively. Column chart on the right showed the phenotype of heading date of each plant. Bars with different colors represent different genotype as legends indicated below. e Protein structure and detailed mutation of OsPRR37 in lhd7 mutant. Bars with different colors represent different protein domains of OsPRR37 as indicated. Numbers near the bars represent the position of amino acid. f Conservation of pseudo-receiver (PR) domain of OsPRR37. Aliments of PR domain of Pseudo responsive regulator (PRR) proteins from different organisms. Red arrow indicates the amino acid Gly (G) at position 159 which mutated to Asp (D) in osprr37. g-h Phenotype of heading date and yield per plant of complementary lines compared with ZH11 and lhd7 mutant