Fig. 5

Spatiotemporal expression and subcellular localization of OsPORR1. a Expression of OsPORR1 in various plant organs, developing seeds and seedlings at different times. DAG, days after germination. Three biological replicates were subjected to qRT-PCR. OsActin1 was used as an internal control for data normalization. The values are the means ± SDs. b Subcellular localization of OsPORR1. GFP served as a control. GFP signals in protoplasts isolated from positive transgenic seedlings were observed via confocal laser scanning microscopy. Mito Tracker Orange was used to identify mitochondria. Bars, 10 μm