Fig. 3

Positional cloning of the Fse5 locus. a The Fse5 locus was restricted to a 54 kb region flanked by markers wi-10 and wi-11 on chromosome 9 (Chr. 9) and included nine ORFs. Both markers and numbers of recombinants are shown. b Mutation site in LOC_Os09g29760 in the fse5 mutant. A deleted 408 bp fragment near the end of the exon leads to a 136 aa truncation in the putative encoded protein in the fse5 mutant. The red letters and dotted lines represent the deletion in LOC_Os09g29760 or its encoded protein in the fse5 mutant. The yellow and orange boxes indicate the 5′ untranslated region (UTR) and single exon of LOC_Os09g29760, respectively. c Expression of LOC_Os09g29760 in WT, fse5 mutant and complemented (COM) lines. The primer pair designed within the 408 bp deletion was used in qRT-PCR. Three biological replicates were subjected to qRT-PCR. OsActin1 was used as an internal control for data normalization. The values are the means ± SDs. ND, not detected. d Phenotypes of seeds (upper panel) and transverse sections (lower panel) of WT, fse5 mutant and COM seeds. Bars, 1 mm. e Nine-day-old seedlings of WT, fse5 mutant and COM lines. Bar, 5 cm