From: Proteomics and Metabolomics Studies on the Biotic Stress Responses of Rice: an Update
Method | Bacteria | Cultivars | Key finding | Reference |
LC-TOF-MS and GC-TOF-MS | Xoo PXO99 and ∆PXO994rax- ST | TP309 (S) and TP309_Xa21 | Alkaloid biosynthesis was increased specifically in TP309_Xa21 to PX099 but not ∆PXO994rax- ST | Sana et al. 2010a |
RP-HPLC-MS | Azospirillum strains 4B and B510 | Cigalon and NPB | Phenolic compounds were mainly affected | Chamam et al. 2013 |
LC-MS | Burkholderia glumae AU6208 and Escherichia coli B6 | Cigalon and NPB treated with Azospirillum lipoferum 4B | Flavonoid compounds and hydroxycinnamic acid (HCA) derivatives changed differently upon each bacterium | Chamam et al. 2015 |
LC-MS/MS | Xoo | Basmati 385 treated with Pseudomonas aeruginosa BRp3 | Rice defense-related enzymes were activated by P. aeruginosa | Yasmin et al. 2017 |
HPLC | Pseudomonas putida RRF3 | TKM 9 | P. putida stimulated plant defense responses and altered rhizosphere chemical constituents | Kandaswamy et al. 2019 |
UHPLC-QE Orbitrap/MS | Bacillus pumilus LZP02 | Longgeng 46 | Bacillus pumilus enhanced carbohydrate metabolism and phenylpropanoid biosynthesis | Liu et al. 2020 |
UHPLC-DAD/ESI-QTOF | 10 PGPR strains and B. glumae AU6208 | NPB | Common metabolomics signature of nine compounds as rice response to different PGPR | Valette et al. 2020 |
Method | Fungi | Cultivars | Key finding | Reference |
FIE-MS, GC-TOF-MS | M. oryzae strain Guy11 | B. distachyon ABR1, H. vulgare Golden Promise and CO39 | Common metabolic re-programming strategy was deployed by M. oryzae in different hosts | Parker et al. 2009 |
HPLC-MS/MS | Fusarium fujikuroi strain VE13 | Dorella (S) and Selenio (R) | Sakuranetin accumulated in resistant cultivar | Siciliano et al. 2015a |
GC-MS | Harpophora oryzae strain R5–6-1 and M. oryzae strain Guy11 | CO39 | Different induction patterns of metabolites of the shikimate and lignin against pathogenic and mutualistic fungi | Xu et al. 2015 |
GC-MS | R. solani | Narayan with and without Bacillus amyloliquefaciens (SN13) treatment | Identified novel aspect of rare sugar induced by Bacillus amyloliquefaciens | Srivastava et al. 2016 |
CE/TOF-MS | R. solani AG-1 isolate C-154 | 29S (S) and 32R (R) | Canavanine was significantly higher in resistant rice | Suharti et al. 2016aa |
CE/TOF-MS | R. solani AG-1 isolate C-154 | 29S (S) and 32R (R) | Chlorogenic acid specifically induced in resistant rice | Suharti et al. 2016ba |
CE/TOF-MS | R. solani AG-1 isolate C-154 | 29S (S) and 32R (R) | Distinct responses of susceptible and resistant rice | Suharti et al. 2016ca |
GC-MS | R. solani AG1-IA isolate BRS1 | PB1 (S) | Altered carbon metabolism and perturbed hormonal signaling | Ghosh et al. 2017 |
2-DE, MALDI-TOF MS/MS & GC-MS | R. solani isolate AGI-IA | IR-64 (WT) and AtNPR1-OX line | Novel immunity-related prognostic proteins induced by AtNPR1 | Karmakar et al. 2019a,b |
QTOF-UPHPLC MS | M. oryzae strain Guy11 | CO39, NPB, and LTH (S); Pi-gm, Pi-4B, and Pi-B (R) | Bayogenin 3-O-Cellobioside, a saponin compound, was first identified in rice for the first time | Norvienyeku et al. 2020a |
Method | Insect | Cultivars | Key finding | Reference |
1H NMR | BPH | TN1 (S) and B5 (R) | Activation of GABA shunt and shikimate metabolisms was vital for BPH resistance | Liu et al. 2010a |
GC-MS | Rice gall midge biotype 1 (GMB1) | TN1, Kavya, and RP2068 | Potential biomarkers of rice-gall midge interaction were identified | Agarrwal et al. 2014a |
GC-MS | GMB1 | RP2068-18-3-5 (R) | During HR, upregulation of LPO and LPO marker metabolite azelaic acid; and higher accumulation of GABA at the feeding site | Agarrwal et al. 2016a |
UHPLC-MS and GC-MS | Rice stem borer (Chilo suppressali) | Minghui 63 | Activation of phytohormones and shikimate-mediated and terpenoid-related secondary metabolism | Liu et al. 2016 |
GC-MS | BPH | TN1 (S) and YHY15 (R) | Resistance to BPH was mediated by SM synthesis through the shikimate pathway | Peng et al. 2016a |
1H NMR and GC-FID/MS | BPH | TN1 (S) and NIL-Bph15 (R) | BPH adapts and recovers at different stage in susceptible and resistant plants | Liu et al. 2017 |
UPLC-Q-TOF MS | BPH | Dongjin treated with B. velezensis YC7010 | B. velezensis induced SA, JA, and secondary metabolites to enhance resistance | Harun-Or-Rashid et al. 2018 |
GC-MS | BPH | NPB (S) and Bph6-transgenic line R6 (R) | Bph6 resistance gene affected lipid levels in leaf sheath only | Zhang et al. 2018a |
GC-MS & LC-MS | BPH | TN1 (S), IR36 and IR56 (R) | Defense-related metabolites, cyanoamino acids, and lipid metabolism were increased by BPH and were more stable in resistant cultivars | Kang et al. 2019a |
UPLC-QToF-MS | BPH | KDML105 (S) and IL308 (R) | Susceptible and resistant rice induced common SMs at different levels | Uawisetwathana et al. 2019a |
LC-ESI-MS/MS | Cnaphalocrocis medinalis | Minghui 63 | JA-dependent signaling pathway was found vital in response to leaf folder | Wang et al. 2020 |
Method | Nematode | Cultivars | Key finding | Reference |
HPLC | Ditylenchus angustus | Two susceptible and five resistant cultivars | Induction and accumulation of phenolic compounds in the resistant varieties | Gill et al. 1996a |
Method | Others | Cultivars | Key finding | Reference |
LC-MS and Q-TOF MS/MS | NA | ZH17 (WT), wrky62, wrky76 and dsOW62/76 | SA, JA, and phenolamides were increased and free pools of flavonoids were decreased in the double mutant | Liang et al. 2017 |