Skip to main content

Table 2 Global metabolomics studies investigating biotic stress responses in rice

From: Proteomics and Metabolomics Studies on the Biotic Stress Responses of Rice: an Update

Method

Bacteria

Cultivars

Key finding

Reference

LC-TOF-MS and GC-TOF-MS

Xoo PXO99 and ∆PXO994rax- ST

TP309 (S) and TP309_Xa21

Alkaloid biosynthesis was increased specifically in TP309_Xa21 to PX099 but not ∆PXO994rax- ST

Sana et al. 2010a

RP-HPLC-MS

Azospirillum strains 4B and B510

Cigalon and NPB

Phenolic compounds were mainly affected

Chamam et al. 2013

LC-MS

Burkholderia glumae AU6208 and Escherichia coli B6

Cigalon and NPB treated with Azospirillum lipoferum 4B

Flavonoid compounds and hydroxycinnamic acid (HCA) derivatives changed differently upon each bacterium

Chamam et al. 2015

LC-MS/MS

Xoo

Basmati 385 treated with Pseudomonas aeruginosa BRp3

Rice defense-related enzymes were activated by P. aeruginosa

Yasmin et al. 2017

HPLC

Pseudomonas putida RRF3

TKM 9

P. putida stimulated plant defense responses and altered rhizosphere chemical constituents

Kandaswamy et al. 2019

UHPLC-QE Orbitrap/MS

Bacillus pumilus LZP02

Longgeng 46

Bacillus pumilus enhanced carbohydrate metabolism and phenylpropanoid biosynthesis

Liu et al. 2020

UHPLC-DAD/ESI-QTOF

10 PGPR strains and B. glumae AU6208

NPB

Common metabolomics signature of nine compounds as rice response to different PGPR

Valette et al. 2020

Method

Fungi

Cultivars

Key finding

Reference

FIE-MS, GC-TOF-MS

M. oryzae strain Guy11

B. distachyon ABR1, H. vulgare Golden Promise and CO39

Common metabolic re-programming strategy was deployed by M. oryzae in different hosts

Parker et al. 2009

HPLC-MS/MS

Fusarium fujikuroi strain VE13

Dorella (S) and Selenio (R)

Sakuranetin accumulated in resistant cultivar

Siciliano et al. 2015a

GC-MS

Harpophora oryzae strain R5–6-1 and M. oryzae strain Guy11

CO39

Different induction patterns of metabolites of the shikimate and lignin against pathogenic and mutualistic fungi

Xu et al. 2015

GC-MS

R. solani

Narayan with and without Bacillus amyloliquefaciens (SN13) treatment

Identified novel aspect of rare sugar induced by Bacillus amyloliquefaciens

Srivastava et al. 2016

CE/TOF-MS

R. solani AG-1 isolate C-154

29S (S) and 32R (R)

Canavanine was significantly higher in resistant rice

Suharti et al. 2016aa

CE/TOF-MS

R. solani AG-1 isolate C-154

29S (S) and 32R (R)

Chlorogenic acid specifically induced in resistant rice

Suharti et al. 2016ba

CE/TOF-MS

R. solani AG-1 isolate C-154

29S (S) and 32R (R)

Distinct responses of susceptible and resistant rice

Suharti et al. 2016ca

GC-MS

R. solani AG1-IA isolate BRS1

PB1 (S)

Altered carbon metabolism and perturbed hormonal signaling

Ghosh et al. 2017

2-DE, MALDI-TOF MS/MS & GC-MS

R. solani isolate AGI-IA

IR-64 (WT) and AtNPR1-OX line

Novel immunity-related prognostic proteins induced by AtNPR1

Karmakar et al. 2019a,b

QTOF-UPHPLC MS

M. oryzae strain Guy11

CO39, NPB, and LTH (S); Pi-gm, Pi-4B, and Pi-B (R)

Bayogenin 3-O-Cellobioside, a saponin compound, was first identified in rice for the first time

Norvienyeku et al. 2020a

Method

Insect

Cultivars

Key finding

Reference

1H NMR

BPH

TN1 (S) and B5 (R)

Activation of GABA shunt and shikimate metabolisms was vital for BPH resistance

Liu et al. 2010a

GC-MS

Rice gall midge biotype 1 (GMB1)

TN1, Kavya, and RP2068

Potential biomarkers of rice-gall midge interaction were identified

Agarrwal et al. 2014a

GC-MS

GMB1

RP2068-18-3-5 (R)

During HR, upregulation of LPO and LPO marker metabolite azelaic acid; and higher accumulation of GABA at the feeding site

Agarrwal et al. 2016a

UHPLC-MS and GC-MS

Rice stem borer (Chilo suppressali)

Minghui 63

Activation of phytohormones and shikimate-mediated and terpenoid-related secondary metabolism

Liu et al. 2016

GC-MS

BPH

TN1 (S) and YHY15 (R)

Resistance to BPH was mediated by SM synthesis through the shikimate pathway

Peng et al. 2016a

1H NMR and GC-FID/MS

BPH

TN1 (S) and NIL-Bph15 (R)

BPH adapts and recovers at different stage in susceptible and resistant plants

Liu et al. 2017

UPLC-Q-TOF MS

BPH

Dongjin treated with B. velezensis YC7010

B. velezensis induced SA, JA, and secondary metabolites to enhance resistance

Harun-Or-Rashid et al. 2018

GC-MS

BPH

NPB (S) and Bph6-transgenic line R6 (R)

Bph6 resistance gene affected lipid levels in leaf sheath only

Zhang et al. 2018a

GC-MS & LC-MS

BPH

TN1 (S), IR36 and IR56 (R)

Defense-related metabolites, cyanoamino acids, and lipid metabolism were increased by BPH and were more stable in resistant cultivars

Kang et al. 2019a

UPLC-QToF-MS

BPH

KDML105 (S) and IL308 (R)

Susceptible and resistant rice induced common SMs at different levels

Uawisetwathana et al. 2019a

LC-ESI-MS/MS

Cnaphalocrocis medinalis

Minghui 63

JA-dependent signaling pathway was found vital in response to leaf folder

Wang et al. 2020

Method

Nematode

Cultivars

Key finding

Reference

HPLC

Ditylenchus angustus

Two susceptible and five resistant cultivars

Induction and accumulation of phenolic compounds in the resistant varieties

Gill et al. 1996a

Method

Others

Cultivars

Key finding

Reference

LC-MS and Q-TOF MS/MS

NA

ZH17 (WT), wrky62, wrky76 and dsOW62/76

SA, JA, and phenolamides were increased and free pools of flavonoids were decreased in the double mutant

Liang et al. 2017

  1. Abbreviations: single asterisk “a” indicates comparative metabolomics studies of resistant and susceptible plants; “b” indicates the study applied both proteomics and metabolomics approaches; Xoo Xanthomonas oryzae pv. oryzae, S Susceptible, R Resistant, WT Wild type, OX Over-expression, NPB Nipponbare, PB1 Pusa Basmati1, PGPR Plant growth promoting rhizobacteria, B. glumae Burkholderia glumae, M. oryzae Magnaporthe oryzae, B. distachyon Brachypodium distachyon, H. vulgare Hordeum vulgare, SA Salicylic acid, JA Jasmonic acid, BPH Brown plant hopper, TN1 Taichung Native-1, HR Hypersensitive response, LPO Lipid peroxidation, SM Secondary metabolite NIL Near-isogenic line, B. velezensis Bacillus velezensis, LMM Lesion mimic mutant, dsOW62/76 Mutant containing RNA interfering constructs of OsWRKY62 and OsWRKY76, 2-DE Two-dimensional gel electrophoresis, MS Mass spectrometry, MALDI-TOF Matrix-assisted laser desorption Ionization time of flight, TOF Time-of-Flight, Q-TOF Quadrupole time-of-flight, LC-MS Liquid chromatography mass spectrometry, HPLC High-performance liquid chromatography, RP-HPLC Reverse phase-high performance liquid chromatography, UHPLC Ultra high performance liquid chromatography, GC-MS Gas chromatography mass spectrometry, CE-MS Capillary electrophoresis mass spectrometry, 1H NMR Proton nuclear magnetic resonance, FID Flame-ionization detection, DAD Diode array detector, NA Not applicable