Skip to main content

Table 1 Global proteomics studies investigating biotic stress responses in rice

From: Proteomics and Metabolomics Studies on the Biotic Stress Responses of Rice: an Update

Method Bacteria Cultivars Key finding Reference
2-DE, MALDI-TOF MS Xoo races T7174 (IC) and Xo7435 (C) Java 14 Thaumatin-like protein (PR5) and probenazole (PBZ) were triggered by JA Mahmood et al. 2006
2-DE, MALDI-TOF/TOF MS Xoo race PXO99A (IC) and DY89031 (C) Xa21-transgenic suspension cells Nine putative PM-associated proteins with potential functions in rice defense were identified Chen et al. 2007
2-DE, MALDI-TOF-TOF MS Xoo strain Zhe173 (IC) somatic hybrid line SH76 (R) Majority of DEPs were involved in photosynthesis Yu et al. 2008
2-DE, NanoLC MS/MS P. fluorescens strain KH-1 Co43 P. fluorescens modulated rice metabolic pathways including energy metabolism and defense Kandasamy et al. 2009
2-DE, MALDI-TOF MS Xoo race Xo7435 Thaumatin-like protein gene transgenic-OX line Variation in oxidative stress and energy metabolism associated proteins was observed in disease resistance Mahmood et al. 2009a
2-DE, MALDI-TOF MS Xoo races T7174 (IC) and Xo7435 (C) Java 14 treated with probenazole PR5 was highly induced in PBZ pretreated plants during their interaction with Xoo Mahmood et al. 2009b
2-DE, MALDI-TOF MS Sinorhizobium meliloti 1021 NPB Defense related proteins were highly induced in root, whereas photosynthesis related proteins induced in leaf Chi et al. 2010
LC-MALDI-MS/MS Xoo XKK.12 Baldo Virulence- associated factors were identified González et al. 2012
2-DE, MALDI-TOF-MS Xoo strain 89,773–1-1 9311 Disease resistance signal transduction, pathogenesis, and regulation of cell metabolism were activated Li et al. 2012a
2-DE, MALDI-TOF MS and nESI-LC-MS/MS Xoo strain K3 Dongjin DUF26, β-1,3-glucanase, and basic secretory protein family proteins as the main host defense related proteins Wang et al. 2013
LC-MS/MS Xoo strain Zhe173 IRBB5 (R) Several epigenetic factors regulated plant disease resistance pathway by alternating phosphorylation and dephosphorylation Hou et al. 2015
2-DE and MALDI-TOF MS Xoo isolate DX133 PB1 (S) and O. longistaminata (R) Proteins related to defense response were mainly found expressed in the resistant plants Kumar et al. 2015
2D-DIGE, MALDI-TOF-MS Xoo strain PXO124 O. meyriana Peroxidase was critical in the early response of O. meyriana Chen et al. 2016
Method Fungi Cultivars Key finding Reference
2-DE, MALDI-TOF-MS M. oryzae race KJ401 (IC) and KJ101 (C) Jinheung Receptor-like protein kinases, pathogenesis-related proteins, and JA were induced in incompatible interaction Sun et al. 2004
2-DE, ESI Q-TOF MS Rhizoctonia solani strain LR 172 Labelle (S) and LSBR-5 (R) 3- β-hydroxysteroid dehydrogenase/isomerase was first identified in resistant rice Lee et al. 2006
2DE, MALDI-TOF MS M. oryzae isolate Hoku1 ZTS (S) and ZTR (R, carryig Pi-zt) Whole plant-specific resistance was associated with thaumatin-like protein Koga et al. 2012
2-DE, MALDI-TOF/TOF MS M. oryzae race ZC13 isolate 97-151a CO39 (S) and C101LAC (R) Resistant cultivar was more sensitive to SA signaling system Li et al. 2012b
2-DE, MALDI-TOF-MS or nESI-LC-MS/MS M. oryzae race KJ401 and KJ301 Jinheung Different defense responses of rice and pathogenicity of M. oryzae Kim et al. 2013
2-DE, MALDI-TOF/TOF-MS and nESI-LC-MS/MS Cochliobolus miyabeanus strain SHS-2 Dongjin Enzymes involved in the Calvin cycle and glycolysis were decreased; but the TCA cycle, amino acids, and ethylene biosynthesis were increased Kim et al. 2014a
2-DE, MALDI-TOF/TOF and nanoLC-MS/MS M. oryzae race ZC13 CO39 (S) and C101LAC (R) Resistant rice had more and rapid signal transduction cascades Li et al. 2015
iTRAQ, LC-MS/MS M. oryzae ZHONG-10-8-14 NPB Activation of ABA signaling in the early stage of infection, but CK signaling in later stages of infection Cao et al. 2016
2-DE, MALDI-ToF R. solani (WGL-12-1) of AG-1 IA Four susceptible and two tolerant cultivars Novel factors associated with susceptibility and resistance Prathi et al. 2018
iTRAQ, HPLC-MS/MS M. oryzae isolates KJ201 and RB22 NPB (WT) and NPB-Piz-t Seven common proteins induced by Piz-t compatible and incompatible interactions Tian et al. 2018
2-DE, MS/MS M. oryzae 14 rice varieties Functional correlation between nuclear reprogramming and immune response during blast disease Narula et al. 2019
iTRAQ, LC-MS/MS R. solani isolate AG1 IA Lemont (S) and Teqing (R) A network of SA, JA, ROS, and the TCA cycle related proteins conferred resistance against R. solani Ma et al. 2020a
iTRAQ, HPLC-MS/MS M. oryzae isolates Guy-11 and YN716 VP-1636 (WT) and GN-5 (pi21-mutant) JA, SA, and ethylene metabolisms were upregulated in mutant line Nawaz et al. 2020
2-DE, LC-MS/MS M. oryzae (race 007.0), and its elicitor chitin NPB and suspension cell derived from NPB calli Rapid alkalinization factors, phytosulfokines, and novel immune response peptide were identified Wang et al. 2020
Method Virus Cultivars Key finding Reference
2-DE, MALDI-TOF/TOF-MS Rice black-streaked dwarf virus Huai 5 (S) Overaccumulation of H2O2 disrupted photosynthesis and metabolism and caused oxidative stress and abnormal plant growth Xu et al. 2013
2-DE, MALDI-TOF MS Rice stripe virus (RSV) Wuyujing 3 (S) and Xudao 3 (R) Downregulation of heat shock protein, protein disulfide isomerase, glyoxalase in Wuyuing 3 Yang et al. 2013
iTRAQ and RP-HPLC, LC-MS/MS RSV Wuyujing 3 Changes of chlorosis, cell death and plant defense by RSV Wang et al. 2015
1-DE, LC-MS/MS Southern rice black-streaked dwarf virus NPB treated with cytosinpeptidemycin PR and HSP were triggered by cytosinpeptidemycin Yu et al. 2018
Method Insect Cultivars Key finding Reference
iTRAQ, nano-LC ESI QqTOF MS BPH, Nilaparvata lugens Stål) TN1 (S) and TN1 carrying Bph15 (R) Glycine cleavage system protein was upregulated in the resistant lines. Wei et al. 2009
2D-DIGE; and 2-DE, MALDI TOF/TOF-MS SBPH, Laodelphax striatellus Fallén) Rice lines 02428 (S) and Pf9279–4 (R) ROS scavenging and SA-mediated SAR were more active in resistant rice Dong et al. 2017
iTRAQ and LC-MS/MS BPH PS (Indica, S), PR (O. officinalis, R), and their hybrid line HR SMs, carbon metabolism, and glyoxylate and dicarboxylate metabolism were key markers of resistance Zhang et al. 2019
nano-LC-MS/MS Cnaphalocrocis medinalis TN1 (S) and Qingliu (R) Phenylalanine ammonia lyase and chalcone synthase were higher in resistant rice Cheah et al. 2020
iTRAQ and nano-LC-MS/MS BPH Biotype I and Biotype Y TN1 (S) and YHY15 (moderately-R) Post-translational modifications, protein turnover, and chaperones presented a significant difference between two BPH biotypes Zha and You 2020
Method Nematode Cultivars Key finding Reference
HPLC-MS/MS Meloidogyne graminicola NPB and Khao Pahk Maw α-linolenic acid, glutathione, and phenylpropanoid biosynthesis were involved in resistance Xiang et al. 2020
Method Others Cultivars Key finding Reference
2-DE, MS/MS NA Kinmaze (WT) and LMM cdr2 Active metabolic changes were associated with programmed cell death Tsunezuka et al. 2005
2-DE, MALDI-TOF-MS NA LMMs spl1 PBZ1 served as a cell death marker and defense protein Kim et al. 2008
2-DE, MALDI-TOF/TOF NA Zhefu802 (WT) and LMM spl5 Defense response was induced, and amino acid metabolism and photosynthesis were reduced in spl5 Chen et al. 2013
2-DE, MALDI-TOF/TOF MS NA CO39 (S) and C101LAC (R) treated with Me-JA MeJA induced higher ROS in resistant rice Li et al. 2014
LC-MS/MS NA ZH11 (WT) and LMM oscul3a Differentially expressed proteins were chloroplast and cytoplasm proteins Gao et al. 2019
2-DE, MALDI-TOF/TOF MS and NanoLC-MS/MS NA CO39 (S) and C101LAC (R, carrying Pi-1 gene) treated with SA Phosphorylation regulation by SA contributed differently in resistant and susceptible rice Sun et al. 2019
  1. Abbreviations: Xoo Xanthomonas oryzae pv. oryzae, IC Incompatible, C Compatible, PM Plasma membrane, S Susceptible, R Resistant, DEP Differentially expressed proteins, P. fluorescens Pseudomonas fluorescens, OX Over-expression, NPB Nipponbare, WT Wild type, PB1 Pusa Basmati1, M. oryzae Magnaporthe oryzae, ABA Abscisic acid, CK Cytokinin, SA Salicylic acid, JA Jasmonic acid, ROS Reactive oxygen species, HSP Heat shock protein, TCA Tricarboxylic acid, BPH Brown plant hopper, TN1 Taichung Native-1, SM Secondary metabolite, SBPH Small brown planthopper, SAR Systemic acquired resistance, LMM Lesion mimic mutant, 1-DE One-dimensional gel electrophoresis, 2-DE Two-dimensional gel electrophoresis, 2D-DIGE Two-dimensional difference gel electrophoresis, MS Mass spectrometry, MALDI-TOF Matrix-assisted laser desorption ionization time of flight, TOF Time-of-Flight, Q-TOF Quadrupole time-of-flight, QqTOF Quadrupole-quadrupole-time-of-flight, iTRAQ Isobaric tags relative and absolute quantification, LC-MS Liquid chromatography mass spectrometry, HPLC High-performance liquid chromatography, RP-HPLC Reverse phase-high performance liquid chromatography, UHPLC Ultra high performance liquid chromatography, GC-MS Gas chromatography mass spectrometry, CE-MS Capillary electrophoresis mass spectrometry, nESI Nano-electrospray ionization source, NA Not applicable