Table 1 Global proteomics studies investigating biotic stress responses in rice
From: Proteomics and Metabolomics Studies on the Biotic Stress Responses of Rice: an Update
Method | Bacteria | Cultivars | Key finding | Reference |
2-DE, MALDI-TOF MS | Xoo races T7174 (IC) and Xo7435 (C) | Java 14 | Thaumatin-like protein (PR5) and probenazole (PBZ) were triggered by JA | Mahmood et al. 2006 |
2-DE, MALDI-TOF/TOF MS | Xoo race PXO99A (IC) and DY89031 (C) | Xa21-transgenic suspension cells | Nine putative PM-associated proteins with potential functions in rice defense were identified | Chen et al. 2007 |
2-DE, MALDI-TOF-TOF MS | Xoo strain Zhe173 (IC) | somatic hybrid line SH76 (R) | Majority of DEPs were involved in photosynthesis | Yu et al. 2008 |
2-DE, NanoLC MS/MS | P. fluorescens strain KH-1 | Co43 | P. fluorescens modulated rice metabolic pathways including energy metabolism and defense | Kandasamy et al. 2009 |
2-DE, MALDI-TOF MS | Xoo race Xo7435 | Thaumatin-like protein gene transgenic-OX line | Variation in oxidative stress and energy metabolism associated proteins was observed in disease resistance | Mahmood et al. 2009a |
2-DE, MALDI-TOF MS | Xoo races T7174 (IC) and Xo7435 (C) | Java 14 treated with probenazole | PR5 was highly induced in PBZ pretreated plants during their interaction with Xoo | Mahmood et al. 2009b |
2-DE, MALDI-TOF MS | Sinorhizobium meliloti 1021 | NPB | Defense related proteins were highly induced in root, whereas photosynthesis related proteins induced in leaf | Chi et al. 2010 |
LC-MALDI-MS/MS | Xoo XKK.12 | Baldo | Virulence- associated factors were identified | González et al. 2012 |
2-DE, MALDI-TOF-MS | Xoo strain 89,773–1-1 | 9311 | Disease resistance signal transduction, pathogenesis, and regulation of cell metabolism were activated | Li et al. 2012a |
2-DE, MALDI-TOF MS and nESI-LC-MS/MS | Xoo strain K3 | Dongjin | DUF26, β-1,3-glucanase, and basic secretory protein family proteins as the main host defense related proteins | Wang et al. 2013 |
LC-MS/MS | Xoo strain Zhe173 | IRBB5 (R) | Several epigenetic factors regulated plant disease resistance pathway by alternating phosphorylation and dephosphorylation | Hou et al. 2015 |
2-DE and MALDI-TOF MS | Xoo isolate DX133 | PB1 (S) and O. longistaminata (R) | Proteins related to defense response were mainly found expressed in the resistant plants | Kumar et al. 2015 |
2D-DIGE, MALDI-TOF-MS | Xoo strain PXO124 | O. meyriana | Peroxidase was critical in the early response of O. meyriana | Chen et al. 2016 |
Method | Fungi | Cultivars | Key finding | Reference |
2-DE, MALDI-TOF-MS | M. oryzae race KJ401 (IC) and KJ101 (C) | Jinheung | Receptor-like protein kinases, pathogenesis-related proteins, and JA were induced in incompatible interaction | Sun et al. 2004 |
2-DE, ESI Q-TOF MS | Rhizoctonia solani strain LR 172 | Labelle (S) and LSBR-5 (R) | 3- β-hydroxysteroid dehydrogenase/isomerase was first identified in resistant rice | Lee et al. 2006 |
2DE, MALDI-TOF MS | M. oryzae isolate Hoku1 | ZTS (S) and ZTR (R, carryig Pi-zt) | Whole plant-specific resistance was associated with thaumatin-like protein | Koga et al. 2012 |
2-DE, MALDI-TOF/TOF MS | M. oryzae race ZC13 isolate 97-151a | CO39 (S) and C101LAC (R) | Resistant cultivar was more sensitive to SA signaling system | Li et al. 2012b |
2-DE, MALDI-TOF-MS or nESI-LC-MS/MS | M. oryzae race KJ401 and KJ301 | Jinheung | Different defense responses of rice and pathogenicity of M. oryzae | Kim et al. 2013 |
2-DE, MALDI-TOF/TOF-MS and nESI-LC-MS/MS | Cochliobolus miyabeanus strain SHS-2 | Dongjin | Enzymes involved in the Calvin cycle and glycolysis were decreased; but the TCA cycle, amino acids, and ethylene biosynthesis were increased | Kim et al. 2014a |
2-DE, MALDI-TOF/TOF and nanoLC-MS/MS | M. oryzae race ZC13 | CO39 (S) and C101LAC (R) | Resistant rice had more and rapid signal transduction cascades | Li et al. 2015 |
iTRAQ, LC-MS/MS | M. oryzae ZHONG-10-8-14 | NPB | Activation of ABA signaling in the early stage of infection, but CK signaling in later stages of infection | Cao et al. 2016 |
2-DE, MALDI-ToF | R. solani (WGL-12-1) of AG-1 IA | Four susceptible and two tolerant cultivars | Novel factors associated with susceptibility and resistance | Prathi et al. 2018 |
iTRAQ, HPLC-MS/MS | M. oryzae isolates KJ201 and RB22 | NPB (WT) and NPB-Piz-t | Seven common proteins induced by Piz-t compatible and incompatible interactions | Tian et al. 2018 |
2-DE, MS/MS | M. oryzae | 14 rice varieties | Functional correlation between nuclear reprogramming and immune response during blast disease | Narula et al. 2019 |
iTRAQ, LC-MS/MS | R. solani isolate AG1 IA | Lemont (S) and Teqing (R) | A network of SA, JA, ROS, and the TCA cycle related proteins conferred resistance against R. solani | Ma et al. 2020a |
iTRAQ, HPLC-MS/MS | M. oryzae isolates Guy-11 and YN716 | VP-1636 (WT) and GN-5 (pi21-mutant) | JA, SA, and ethylene metabolisms were upregulated in mutant line | Nawaz et al. 2020 |
2-DE, LC-MS/MS | M. oryzae (race 007.0), and its elicitor chitin | NPB and suspension cell derived from NPB calli | Rapid alkalinization factors, phytosulfokines, and novel immune response peptide were identified | Wang et al. 2020 |
Method | Virus | Cultivars | Key finding | Reference |
2-DE, MALDI-TOF/TOF-MS | Rice black-streaked dwarf virus | Huai 5 (S) | Overaccumulation of H2O2 disrupted photosynthesis and metabolism and caused oxidative stress and abnormal plant growth | Xu et al. 2013 |
2-DE, MALDI-TOF MS | Rice stripe virus (RSV) | Wuyujing 3 (S) and Xudao 3 (R) | Downregulation of heat shock protein, protein disulfide isomerase, glyoxalase in Wuyuing 3 | Yang et al. 2013 |
iTRAQ and RP-HPLC, LC-MS/MS | RSV | Wuyujing 3 | Changes of chlorosis, cell death and plant defense by RSV | Wang et al. 2015 |
1-DE, LC-MS/MS | Southern rice black-streaked dwarf virus | NPB treated with cytosinpeptidemycin | PR and HSP were triggered by cytosinpeptidemycin | Yu et al. 2018 |
Method | Insect | Cultivars | Key finding | Reference |
iTRAQ, nano-LC ESI QqTOF MS | BPH, Nilaparvata lugens Stål) | TN1 (S) and TN1 carrying Bph15 (R) | Glycine cleavage system protein was upregulated in the resistant lines. | Wei et al. 2009 |
2D-DIGE; and 2-DE, MALDI TOF/TOF-MS | SBPH, Laodelphax striatellus Fallén) | Rice lines 02428 (S) and Pf9279–4 (R) | ROS scavenging and SA-mediated SAR were more active in resistant rice | Dong et al. 2017 |
iTRAQ and LC-MS/MS | BPH | PS (Indica, S), PR (O. officinalis, R), and their hybrid line HR | SMs, carbon metabolism, and glyoxylate and dicarboxylate metabolism were key markers of resistance | Zhang et al. 2019 |
nano-LC-MS/MS | Cnaphalocrocis medinalis | TN1 (S) and Qingliu (R) | Phenylalanine ammonia lyase and chalcone synthase were higher in resistant rice | Cheah et al. 2020 |
iTRAQ and nano-LC-MS/MS | BPH Biotype I and Biotype Y | TN1 (S) and YHY15 (moderately-R) | Post-translational modifications, protein turnover, and chaperones presented a significant difference between two BPH biotypes | Zha and You 2020 |
Method | Nematode | Cultivars | Key finding | Reference |
HPLC-MS/MS | Meloidogyne graminicola | NPB and Khao Pahk Maw | α-linolenic acid, glutathione, and phenylpropanoid biosynthesis were involved in resistance | Xiang et al. 2020 |
Method | Others | Cultivars | Key finding | Reference |
2-DE, MS/MS | NA | Kinmaze (WT) and LMM cdr2 | Active metabolic changes were associated with programmed cell death | Tsunezuka et al. 2005 |
2-DE, MALDI-TOF-MS | NA | LMMs spl1 | PBZ1 served as a cell death marker and defense protein | Kim et al. 2008 |
2-DE, MALDI-TOF/TOF | NA | Zhefu802 (WT) and LMM spl5 | Defense response was induced, and amino acid metabolism and photosynthesis were reduced in spl5 | Chen et al. 2013 |
2-DE, MALDI-TOF/TOF MS | NA | CO39 (S) and C101LAC (R) treated with Me-JA | MeJA induced higher ROS in resistant rice | Li et al. 2014 |
LC-MS/MS | NA | ZH11 (WT) and LMM oscul3a | Differentially expressed proteins were chloroplast and cytoplasm proteins | Gao et al. 2019 |
2-DE, MALDI-TOF/TOF MS and NanoLC-MS/MS | NA | CO39 (S) and C101LAC (R, carrying Pi-1 gene) treated with SA | Phosphorylation regulation by SA contributed differently in resistant and susceptible rice | Sun et al. 2019 |