Table 1 List of B and P Donor vectors used to assemble the 5-stack and 11-stack T-DNA
From: Efficient Gene Stacking in Rice Using the GAANTRY System
Stack # | Donor Vector | Phenotype | Cargo Sequence | |||
|---|---|---|---|---|---|---|
Cargo Size (kb) | Promoter | CDSa | Terminator | |||
1 | B | 3.4 | hygromycin resistance | RUBQ2 | hptII | CaMV 35S |
2 | P | 2.1 | Renilla luciferase activity | CaMV 35S | Rluc | CaMV 35S |
3 | B | 2.2 | enhancer blocking insulator | TBS (Transformation Booster Sequence) | ||
4 | P | 4.2 | firefly luciferase activity | OsCc1 | Fluc | nos |
5 | B | 4.8 | β-glucuronidase activity | OsLP2 | GUSPlus | nos |
6 | P | 3.1 | glufosinate herbicide tolerance | PvUbi1 | bar | nos |
7 | B | 3.0 | green florescence | OsRoot6 | eGFP | CaMV 35S |
8 | P | 2.2 | enhancer blocking insulator | TBS (Transformation Booster Sequence) | ||
9 | B | 4.3 | red florescenceb | OsPS2 | tdTomatoER | nos/35S |
10 | P | 4.6 | glyphosate herbicide tolerance | OsGOS2 | EPSPS | nos |
11 | B | 3.4 | paromomycin resistance | ZmUbi1 | nptII | CaMV 35S |