Fig. 4

The GAANTRY 5-stack T-DNA and summary of the phenotypes and genotypes of the independent events. The structure of the 16.9 kb 5-stack GAANTRY T-DNA (top). PCR amplicons used to determine the presence of the T-DNA cargo sequences and the LB backbone sequence are indicated by the numbered rectangles below the T-DNA diagram. Amplicon 5* is fully within the GUSPlus cargo sequence, while the other amplicons span the junction between cargo sequences. The distance of each complete cargo sequence is from the right border (in kb) is shown below the diagram. Colored rectangles indicate the presence of the associated phenotype and/or genotype for each independent transgenic event. The asterisks (*) mark the events with a single copy of the hptII and GUSPlus transgenes. The plus sign (+) marks the event that contained sequence outside of the T-DNA beyond the left border