Fig. 2
From: Efficient Gene Stacking in Rice Using the GAANTRY System
Composition of the 11-stack T -DNA and PCR validation of cargo sequences. a Diagram of the GAANTRY 11-stack 37.4 kb T-DNA. See Fig. 1 legend for a description of the diagram. PCR products that bridge junctions between each cargo sequence are indicated by the numbered rectangles below the T-DNA. b Gel electrophoresis image of the PCR amplicons spanning each junction. Abbreviations are as follows: hptII (hygromycin phosphotransferase 2), Rluc (Renilla luciferase), TBS (Transformation Booster Sequence), Fluc (Firefly luciferase), GUSPlus (β-glucuronidase encoding reporter gene), bar (bialaphos resistance), eGFP (enhanced Green Fluorescent Protein), tdTom (tdTomato red fluorescent protein), EPSPS (5-enol-pyruvylshikimate-3-phospate synthase), and nptII (neomycin phosphotransferase 2)