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Fig. 6 | Rice

Fig. 6

From: A Semi-Dominant Mutation in OsCESA9 Improves Salt Tolerance and Favors Field Straw Decay Traits by Altering Cell Wall Properties in Rice

Fig. 6

OsCESA9D387N compete with OsCESA9 for interaction with OsCESA4 and OsCESA7. a Both OsCESA9 and OsCESA9D387N can interact with OsCESA4 and OsCESA7 in yeast. 182-OsCESA9 and 182-OsCESA9D387N are the bait plasmid containing full length cDNA of OsCESA9 and OsCESA9D387N, respectively. 184-OsCESA4 and 184-OsCESA7 are the prey plasmid containing full length cDNA of OsCESA4 and OsCESA7, respectively. 184-Mock, empty prey plasmid. SD/−T -L, SD medium lacking tryptophan and leucine; SD/−T -L -H -A, SD medium lacking tryptophan, leucine, histidine, and adenine. b, c Split firefly luciferase complementation (SPLC) assays showing the interactions of OsCESA9 with OsCESA4 and OsCESA7 were weakened in the presence of OsCESA9D387N in N. benthamiana leaves, Bars = 2 cm. The western blotting results in (b) indicating all constructs were agroinfiltrated into N. benthamiana leaves have been expressed. c The relative luminescence unit (RLU) values were measured by using the red circle (b) for crude enzyme extraction. Different letters denote significant differences (P < 0.05, Duncan’s multiple range test). d BiFC assays indicate that OsCESA9D387N direct interact with OsCESA4 and OsCESA7 on the plasma membrane, consist with its WT form OsCESA9, Bars = 60 μm

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