Fig. 6
From: Decreased Vascular Bundle 1 affects mitochondrial and plant development in rice
Mitochondrial defect of dvb1 and dimer and polymer of DVB1 and DVB1G90E. a, b Transmission electron micrographs of mitochondria of the wild type (a) and the dvb1 mutant (b). c Ratio of normal and abnormal mitochondria in wild type and the dvb1 mutant. d ATP content in wild type and dvb1 leaves. e Self-activation of DVB1 and DVB1G90E in yeast cells. (f) Interaction with themselves and with each other of DVB1and DVB1G90E in yeast cells. g\ Interaction with itself of DVB11–91AA in yeast cells. h Immunoblotting using anti His Tag antibodies after SDS-PAGE (left) showed DVB1 and DVB1G90E expressed and purified from E. coli have the expected size of 34.5 kDa, and after Native-PAGE (right) that DVB1 and DVB1G90E can form dimers and smaller degree of polymers. CW: cell wall; CM: cell membrane; Cy: cytoplasm; Mt: normal mitochondria; abMt: abnormal mitochondria; Values represent means ± SD from 6 biological samples. Asterisks indicate the significance of differences between the wild type and the dvb1 mutant as determined by Student’s t-test (*, 0.01 ≤ P < 0.05; **, P < 0.01). Scale bars: 500 nm (a, b)