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Fig. 2 | Rice

Fig. 2

From: The Fungal Effector Avr-Pita Suppresses Innate Immunity by Increasing COX Activity in Rice Mitochondria

Fig. 2

Avr-Pita interacts with OsCOX11. a Analysis of protein–protein interactions between BD:Avr-Pita (mature protease) and AD:OsCOX11 via yeast-two-hybrid (Y2H) assay. Yeast cells harboring the indicated bait and prey plasmids were diluted 10×, 100×, or 1000× and spotted onto selective media SD-LW and SD-AHLW. b In vitro pull-down assays were conducted with fusion proteins (GST:Avr-Pita and His:OsCOX11) using His-Ni beads and detected by immunoblot analysis with anti-GST and anti-His antibodies. Asterisks indicate the target protein. (c) Deletion assays of OsCOX11 to test interactions with Avr-Pita. A series of truncated OsCOX11 proteins were tested for interaction with Avr-Pita via Y2H

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