Fig. 2
From: Indeterminate Domain Proteins Regulate Rice Defense to Sheath Blight Disease
Interaction between LPA1 and IDD3 or IDD13. aIDD3, IDD5, IDD10, and IDD13 expression levels in the leaves at 0, 24, 48, and 72 h post-inoculation (hpi) of Rhizoctonia solani AG1-IA. The error bars are the mean ± SE (n = 3). The statistical analysis was performed for each gene, and different letters indicate significant differences at P < 0.05. b A yeast two-hybrid assay was performed to analyze the interaction between LPA1 and IDD3, IDD10, or IDD13. BD: GAL4-DNA binding domain; AD: activation domain; −T: without tryptophan; −L: without leucine; −H: without histidine. c Reconstitution of GFP fluorescence from LPA1-nYFP + IDD3-cCFP, LPA1-nYFP + IDD13-cCFP, and LPA1-nYFP + cCFP. Bars = 10 μm. DIC: differential interference contrast. d A co-IP assay was performed to analyze the interaction between LPA1 and IDD3, IDD13, or IDD10 in tobacco leaves. IDD3-Myc, IDD13-Myc, IDD10-Myc + LPA1-GFP, or empty vector + LPA1-GFP were transformed into tobacco leaves using Agrobacterium-mediated transformation. Green fluorescent protein (GFP) antibody-immunoprecipitated proteins were analyzed using western blot analysis with the Myc antibody. IDD3-Myc, IDD13-Myc, IDD10-Myc, and LPA1-GFP levels were analyzed by western blot using Myc and GFP antibodies, respectively