Fig. 2
From: Intron-Based Single Transcript Unit CRISPR Systems for Plant Genome Editing
Application of iSTU-CRISPR/Cas9 in rice protoplasts. a Schematic illustration of the iSTU-CRISPR/Cas9 system based on Cas9 protein and intron-spliced sgRNA. The lariat structure of intron splicing is shown. GT (U), AG, intron boundaries. a, branch site. b Quantification of iSTU-CRISPR/Cas9 (inO) induced mutagenesis at OsDEP1 and OsPDS target sites by deep sequencing. The results of three different guide RNA units based on inO were shown. c, d The editing profile of iSTU-CRISPR/Cas9 (inO) at OsDEP1-sgRNA01 target site. The frequencies of deletion at different positions (c) and the total deletion sizes (d) were quantified by deep sequencing. Data for ‘B-D’ are shown as mean ± s.d. (n = 3)