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Fig. 1 | Rice

Fig. 1

From: Intron-Based Single Transcript Unit CRISPR Systems for Plant Genome Editing

Fig. 1

Evaluation of three intron and processing systems with an eGFP reporter in rice protoplasts. a Schematic illustration of intron splicing within the eGFP mRNA. Three introns (inS, inO and inR) are shown with accession number and length. Three guide RNA processing units (tRNA, RZ and NU) are compared. b Representative images of eGFP expressing protoplasts with the inO intron carrying different guide RNA units. eGFP, enhanced green fluorescence protein; ΔeGFP, eGFP with a frame-shift insertion mutation as a negative control. Scale bar = 100 μm. c Quantification of relative fluorescence intensity for eGFP-positive cells among different introns and guide RNA units. Intron-less eGFP was used as a positive control. Data are shown as mean ± s.d. (n = 3). The relative fluorescence intensity was quantified by ImageJ. d Confirmation of intron splicing by PCR amplification of eGFP from plasmid DNA and Complementary DNA (cDNA)

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