Fig. 7
From: MINI SEED 2 (MIS2) Encodes a Receptor-like Kinase that Controls Grain Size and Shape in Rice
Subcellular behavior of MIS and MIS2mu protein. (a) Three-dimensional model of the MIS2 extracellular repeat domain using PyMol. Seven repeats are labeled and Arg 258, which is mutated to Gln in mis2, is shown as a stick and colored purple. (b-e) Co-expression of MIS2 and ARA6 in tobacco leaf. The florescence of MIS2::GFP (b) and ARA6::mCherry (c) were detected and merged on the plasma membrane (d). The merged image (e) is a magnification view of the boxed region in (d). Line arrows indicate GFP-only vesicles, and dot arrows indicate co-localizations. Bar = 20 μm (b-d), Bar = 10 μm (e). (f-i) Co-expression of MIS2mu and ARA6 in tobacco leaf epidermal cells. ARA6::mCherry is localized on plasma membrane (g). MIS2mu::GFP localization is considerably changed (f, h). Magnification view shows GFP-labeled and mCherry-labeled compartments in cytoplasm and no colocalization is observed (I). Line arrows indicate GFP-only vesicles, respectively. Bar = 20 μm (f-h), Bar = 10 μm (i)