Fig. 5
From: MINI SEED 2 (MIS2) Encodes a Receptor-like Kinase that Controls Grain Size and Shape in Rice
Map-Based cloning of MIS2 and genetic complementation. (a) The MIS2 locus was mapped to a region between markers M2 and M3 on chromosome 3. (b, c) The candidate gene was further delimited to a 213-kb genomic region between markers M7 and M8. Twenty-one candidate genes are predicted in this region. The numbers beneath the marker positions indicate the number of recombinants. (d) The MIS2 gene structure. White and green box represent untranslated region and coding sequence, respectively. Black lines represent introns. The start codon (ATG) and the stop codon (TGA) are indicated. A single nucleotide mutation from G to A in MIS2 resulted in an arginine-to-glutamine change. (e) Complementation of mature grain morphology. Three genomic DNA complementation transgenic lines are shown. Bar = 2.5 mm