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Rice

Table 1 The PCR and sequencing results of different haplotypes of 4 R-gene loci in 54 rice varieties. PCR: Polymerase chain reaction; +: PCR positive; −: PCR negative; LTH: Lijiangxintuanheigu

From: Prevalence of Ineffective Haplotypes at the Rice Blast Resistance (R) Gene Loci in Chinese Elite Hybrid Rice Varieties Revealed by Sequence-Based Molecular Diagnosis

Varieties PCR results Sequencing results
Pik Pi5 Pi2/9 Pib Pik Pi5 Pi2/9 Pib
Shanyou 63 – + – + – Pi5–2 – Pib-GII
Zhefu 802 + – + – Pikm1-LTH – Pi2/9-V20 –
Zhe 773 + – + + Pikm1-LTH – Pi2/9-V20 Pib-GII
Wuyugeng No.3 – – – – – – – –
Shanyou 46 – – – + – – – Heterozygote (Pib/Pib-GII)
Gengxian 89 – – – + – – – Pib
Weiyou 46 + – + + Pikm1-LTH – Pi2/9-V20 Heterozygote (Pib/Pib-GII)
Weiyou 77 + + + + Pikm1-LTH Pi5–2 Pi2/9-V20 Heterozygote (Pib/Pib-GII)
Wuyugeng No.2 + + – – Pik-1 Pi5–2 – –
Qishanzhan – + – + – Pi5–2 – Pib
Xiangzaoxian No.7 + – – + Pikm1-LTH – – Pib-GII
Ewan No.5 – – – – – – – –
Eyi 105 – – – + – – – Pib-GII
Xiushui 122 – – – – – – – –
Shenliangyou 5814 – – – + – – – Pib-GII
Y liangyou No.1 – – – + – – – Pib-GII
Wuyou 308 – + + + – Pi5–2 Pi2/9-V20 Pib
Tianyouhuazhan + + + + Pi7–1 Pi5–2 Pi2 Pib
Yangliangyou No.6 + + – + Pikm1-LTH Pi5–2 – Heterozygote (Pib/Pib-GII)
Chuanyou 6203 + + – + Pikm1-TS Pi5–2 – Pib
Gangyou 188 + + – + Pik-1-Shin2 Pi5–2 – Heterozygote (Pib/Pib-GII)
Wuyouhuazhan – + + + – Pi5–2 Pi2 Pib
C liangyouhuazhan + + + + Pikm1-LTH Pi5–2 Heterozygote (Pi2/9-V20/Pi2) Pib
Xinliangyou No.6 – + – + – Pi5–2 – Heterozygote (Pib/Pib-GII)
Y liangyou 5867 – – – + – – – Pib-GII
Zhongzheyou No.8 – + – – – Pi5–2 – –
Zhongzheyou No.1 – + – + – Pi5–2 – Pib
Xinrongyouhuazhan – + + + – Pi5–2 Pi2 Pib
Yueyou 9113 + – – + Pikm1-LTH – – Pib
Rongyou 225 – + + + – Pi5–2 Pi2/9-V20 Heterozygote (Pib/Pib-GII)
Rongyouhuazhan – + + + – Pi5–2 Heterozygote (Pi2/9-V20/Pi2) Heterozygote (Pib/Pib-GII)
Liangyou 6326 – + – + – Pi5–2 – Heterozygote (Pib/Pib-GII)
H you 518 – – – + – – – Pib
Luliangyou 996 + – + + Pikm1-LTH – Pi2/9-V20 Pib-GII
Guangliangyouxiang 66 – – – + – – – Heterozygote (Pib/Pib-GII)
C liangyou 396 + + +   Pikm1-LTH Pi5–2 Pi2/9-V20 –
Wandao153 + – – + Pikm1-LTH – – Pib
Fengliangyou No.4 – + – + – Pi5–2 – Heterozygote (Pib/Pib-GII)
F you 498 + + + + Pikm1-LTH Pi5–2 Piz-t Pib-GII
C liangyou 343 + – + + Pikm1-LTH – Pi2/9-V20 Pib
Yueyou518 – – – + – – – Pib
Wufengyou T025 + – + – Pikm1-LTH – Pi2/9-V20 –
Taiyou 390 + – + + Heterozygote (Pi7–1/Pikm1-LTH) – Pi2/9-V20 Pib
Tanliangyou83 + + + + Pikm1-LTH Pi5–2 Pi50 Pib-GII
Tianfengyou 316 + – + + Heterozygote (Pi7–1/Pikm1-LTH) – Pi2/9-V20 Pib
Y liangyou 9918 – + – – – Pi5–2 – –
C liangyou 608 + – + – Pikm1-LTH – Pi2/9-V20 –
Liangyou 688 – + + – – Pi5–2 Pi2 –
Huiliangyou 996 + + – + Pikm1-LTH Pi5–2 – Pib
Tianyou 998 – – + – – – Pi2/9-V20 –
Zhuliangyou 819 – – + + – – Pi2/9-V20 Pib-GII
Y liangyou No.6 – + – + – Pi5–2 – Pib-GII
Fengyuanyou 299 + – + + Pikm1-LTH – Pi2/9-V20 Heterozygote (Pib/Pib-GII)
Dexiang 4103 + + – + – Pi5–2 – Pib-GII
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