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Table 3 Deletions, insertions, deletions plus insertions (Ind/del) and substitutions generated using SpCAS9, LbCPF1 and BE_nCAS9D10A expressed as % of total mutant alleles identified. A total of 60, 30 and 22 mutants alleles were identified for SpCAS9, LbCPF1 and BE_nCAS9D10A respectively (see also Fig. 3)

From: Beyond Seek and Destroy: how to Generate Allelic Series Using Genome Editing Tools

 

Deletions

Insertions

Ind/del

Substitutions

SpCAS9

27% (16)

52% (31)

21% (13)

0% (0)

LbCPF1

100% (30)

0% (0)

0% (0)

0% (0)

BE_CAS9

9% (2)

0% (0)

0% (0)

91% (20)