Table 1 Homozygous, heterozygous, biallelic and wild-type plants produced using SpCAS9, LbCPF1 and BE_nCAS9D10A expressed as % of analyzed regenerated plants
From: Beyond Seek and Destroy: how to Generate Allelic Series Using Genome Editing Tools
Homozygous | Biallelic | Heterozygous | Wild type | |
|---|---|---|---|---|
SpCAS9 (n = 35) | 37.1% (13) | 45.7% (16) | 11.4% (4) | 5.7% (2) |
LbCPF1 (n = 25) | 12% (3) | 40% (10) | 16% (4) | 28% (7) |
BE_nCAS9D10A (n = 44) | 13.6% (6) | 0% (0) | 22.7% (10) | 63.6% (28) |