Fig. 6
From: OsMS188 Is a Key Regulator of Tapetum Development and Sporopollenin Synthesis in Rice
OsMS188 interacts with TDR. a Amino acid sequence alignment of OsMS188 and AtMS188. The red box indicates the activation domain and the red dots indicate the number of OsMS188 amino acid sequences. b Identification of the activation domain of OsMS188. The full-length CDS of OsMS188 allowed growth on selective media lacking Leu, His and adenine. The yeast could not grow on selective media when the last 14 amino acids were deleted. c Yeast two-hybrid (Y2H) assay showing the physical interaction of OsMS188 with TDR. The OsMS188 CDS without the self-activation fragment (OsMS188ΔC) was fused to the DNA-binding domain (BD) in pGBKT7, and the TDR CDS was fused to the GAL4 activation domain (AD) in pGADT7. The interactions are indicated by blue-coloured yeast colonies on SD/−Ade/−His/−Trp/−Leu/X-a-Gal media. d LCI assay showing the interaction between OsMS188 and TDR. Fluorescence signal intensities indicate interaction activities