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Fig. 3 | Rice

Fig. 3

From: OsNBL1, a Multi-Organelle Localized Protein, Plays Essential Roles in Rice Senescence, Disease Resistance, and Salt Tolerance

Fig. 3

Determination the resistance of nbl1 and WT plants to M. oryzae and Xoo. a-d Punch (a) and spray (b) inoculation of nbl1 and WT plants with a weak virulent M. oryzae isolate 14–856-2. Pictures were photographed at 96 h post inoculation (hpi). 104, 105 and 106 indicate three concentrations of 14–856-2, 1 × 104 spores/mL, 1 × 105 spores/mL and 1 × 106 spores/mL, respectively. Lesion length (c) and lesion number (d) were measured at 96 hpi (1 × 106 spores/mL). Values are means ± SD (n=3). **P < 0.01 (Student’s t-test). e-f Leaves of nbl1 and WT plants were inoculated with Xoo isolate PXO.99. Picture of leaves (e) were photographed and lesion lengths (f) were measured at 14 days post inoculation (dpi). Values are means ± SD (n=10). **P < 0.01 (Student’s t-test). g Observation and record the infection process of 14–856-2 on the sheath cells of nbl1 and WT plants by microscopy at 24 hpi and 48 hpi. DAB staining of infection sites was carried out at 48 hpi. Brown shading indicates accumulation of H2O2

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