Identification of Novel Alleles of the Rice Blast-Resistance Gene Pi9 through Sequence-Based Allele Mining

Rice

Table 5 Disease responses of Pigm, Pi2, Pizt, Pi9 and Pi9 alleles honor plants to M. oryzae isolates

Gene	Varieties	Generation	Enshi		Yichang
Gene	Varieties	Generation	Lr	Nr	Lr	Nr
None	LTH	F0	S	S	HS	S
None	J23B	F0	MS	S	HS	MS
Pigm	GM4H	F0	MR	R	HR	HR
Pi2	C101 A51	F0	R	MR	MR	MR
Pi9	75–1-127	F0	MR	R	HR	R
Pizt	DY1H	F0	MR	MR	R	MR
Pi9-Type1	Heo Trang	F0	HR	R	HR	R
Pi9-Type2	IR64	F0	MS	MS	R	R
Pi9-Type3 ^a	HC1H	F0	R	MR	R	R
Pi9-Type4 ^a	PIIB	F0	R	R	HR	HR
Pi9-Type5 ^a	GD-1S	F0	R	HR	HR	HR
Pi9-Type5-Pi2	CT 18664–9–18-1-3-2	F0	MR	MR	MR	MR
Pi9-Type5-Pizt	ASD 18	F0	MR	MS	MR	MR
Pi9-Type6 ^a	YD4038	F0	R	HR	HR	HR
Pi9-Type7	KAUKKYI ANI	F0	MR	HR	HR	R
Pi9-Type8	DY1H	F0	S	HS	HR	HR
Pi9-Type9 ^a	THAVALU	F0	HR	R	HR	R
Pi9-Type10 ^a	ZWH210	F0	R	HR	HR	MR
Pi9-Type11^a	R03138	F0	HR	HR	HR	HR
Pi9-Type12	JP-5	F0	R	HR	HR	MS
Pi9-Type13	ZD5H	F0	MR	MR	R	HS
Pigm	GM4H	BC3F2	MR	MR	R	HR
Pi2	C101 A51	BC2F2	MR	MS	MR	R
Pi9	75–1-127	BC3F2	MR	MR	R	R
Pizt	DY1H	BC3F2	MR	MS	MR	MR
Pi9-Type3	HC1H	BC3F2	MR	MR	MS	R
Pi9-Type4	PIIB	BC2F2	MR	R	HR	R
Pi9-Type5	GD-1S	BC3F2	MR	MS	R	R
Pi9-Type6	YD4038	BC3F2	R	MR	R	R
Pi9-Type9	THAVALU	BC3F2	MR	MS	R	MR
Pi9-Type10	ZWH210	BC2F2	R	MR	R	MR
Pi9-Type11	R03138	BC3F2	R	MR	R	R

Lr Leaf blast; Nr Ear blast and neck blast; Varieties, Representative donor variety containing the allele
^aWhen Resistance ratio was greater than 85% and Resistance level was less than 2, NILs of allele materials were done
None, no functional Pi9 allele. Pi9-Type5 has three different type Pi9-Type5-Pi2, Pi9-Type5-Piz-t and Pi9-Type5. Donors of Pi9-Type5-Pi2, Pi9-Type5-Piz-t and Pi9-Type5 carried Pi2, Piz-t and no genes, respectively. Donors of Pi9-Type4 carried Pigm gene. Donors of Pi9-Type8 carried Piz-t gene