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Rice

Table 3 Apparent and true amylose contents and short/long amylopectin branch ratios of starch and amylopectin

From: Generation and Starch Characterization of Non-Transgenic BEI and BEIIb Double Mutant Rice (Oryza sativa) with Ultra-High Level of Resistant Starch

Genotype

Fraction I (%)a

III/II ratiob

TAC (%)c

Starch

(AAC)

Amylopectin

(ELC)

Starch

Amylopectin

Starch

WT (Taichung 65)

21.1 ± 0.3f

1.8 ± 0.1de

3.0 ± 0.0d

3.1 ± 0.1d

19.2

WT (Kinmaze)

21.6 ± 2.0f

2.5 ± 0.2d

2.8 ± 0.2d

3.2 ± 0.1d

19.1

be1 (EM557)

21.6 ± 0.3f

2.0 ± 0.1d

3.2 ± 0.0d

3.3 ± 0.1d

19.5

be2b (EM10)

26.5 ± 0.7e

2.0 ± 0.1d

1.0 ± 0.0e

0.9 ± 0.1e

24.5

be1 be2b (#1403)

51.7 ± 0.4d

1.3 ± 0.1e

0.5 ± 0.0f

0.6 ± 0.0e

50.5

  1. aFraction I of starch analyzed by gel filtration chromatography represents the apparent amylose content (AAC), and that of purified amylopectin represents the extra-long chain content (ELC)
  2. bIII/II ratio represents the ratio of amylopectin short chains (fraction III) to amylopectin long chains (Fraction II). Data represent mean ± SE (n = 3). Each fraction was split as shown in Fig. S1
  3. cTrue amylose content (TAC) was calculated by subtracting ELC from AAC
  4. Different lowercase letters (d–f) indicate significant differences among rice genotypes (P < 0.05; Tukey-Kramer method)
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