Fig. 5
From: RICE ACYL-COA-BINDING PROTEIN6 Affects Acyl-CoA Homeostasis and Growth in Rice
Disruption of reactive oxygen species homeostasis in the rice osacbp6 mutant. a Relative expression of genes encoding class III peroxidases. Values are mean ± SD (n = 3). b Comparison of peroxidase activities of the roots and shoots of 7-day-old seedlings and leaves of 21-day-old wild type (WT), osacbp6 and complemented line osacbp6-C4 (C4). Values are means ± SD (n = 3). c Accumulation of superoxide anion (O2∙-) and hydrogen peroxide (H2O2) in the leaf blades of osacbp6. Leaf blades of 21-day-old WT and osacbp6 were subjected to nitroblue tetrazolium (NBT) staining to detect O2∙- or 3,3′-diaminobenzidine tetrahydrochloride (DAB) staining to detect H2O2 in the dark for 3 and 24 h, respectively. Bars, 1 cm. d H2O2 content in the roots and shoots of 7-day-old seedlings and leaves of 21-day-old WT, osacbp6 and C4. Values are means ± SD (n = 3). Asterisks indicate significant differences as evaluated by Student’s t-tests: *P < 0.05, **P < 0.01, ***P < 0.001. WT, Oryza sativa var. japonica cv. Dongjin