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Fig. 5 | Rice

Fig. 5

From: A Cyclophilin OsCYP20–2 Interacts with OsSYF2 to Regulate Grain Length by Pre-mRNA Splicing

Fig. 5

Analysis of RNA-seq between ZH11 and SYF2-RNAi9. a Overview of differentially-expressed genes between ZH11 and OsSYF2-RNAi9 transcriptomes. b Scatter-plot showing the differentially expressed genes between ZH11 and OsSYF2-RNAi9 transcriptomes. c Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analysis of genes expressed in ZH11 and OsSYF2-RNAi9 6 cm panicles. KEGG pathway analyses were applied to genes up-regulated or down-regulated between ZH11 and OsSYF2-RNAi9. Significant enrichment was considered with P value < 0.05. d and e Sucrose (d) and maltose (e) content in the mature endosperm of ZH11, Ri9, and Ri11. Data means SD (n = 3). Letters indicate significant differences determined by a LSD test (P < 0.05). f Starch morphology of ZH11 and OsSYF2 transgenic plants detected by scanning electron microscopy. g The percentage of alternative splicing events between ZH11 and SYF2-RNAi9 revealed by RNA-Seq analysis. Alternative 5′ splice site (A5SS), alternative 3′ splice site (A3SS), mutually exclusive exon (MXE), intron retention (IR), and skipping exon (SE). h Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analysis of differential SE and IR expressed genes between ZH11 and SYF2-RNAi9 transcriptome. Significant enrichment was detected with P value < 0.05

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