Fig. 2
From: A Cyclophilin OsCYP20–2 Interacts with OsSYF2 to Regulate Grain Length by Pre-mRNA Splicing
The interaction between OsCYP20–2 and OsSYF2. a OsCYP20–2 interacted with OsSYF2 in yeast. The clones were cultured in SD/-Trp-Leu-His-Ade medium containing 2 μg/mL X-α-gal. b GST pull-down assay identified the interaction of CYP20–2 with OsSYF2 in vitro. c Subcellular localization of OsSYF2. OsSYF2-GFP was co-expressed with H2B-mCherry in rice protoplasts. Bar = 50 μm. d Expression pattern of OsSYF2. The GUS staining of glume of OsSYF2::GUS transgenic plants (left) various stages of panicle from P2 to P6 stage (Jain et al. 2007) and transcript levels of OsSYF2 at different length of panicles. The panicles with indicated lengths were sampled for the analysis