Fig. 1

Phenotypes of knock-out mutant oscyp20–2 t1. a Comparison of brown grain length between wild type Hwayoung (HY) and oscyp20–2 t1 (cyp). At least 200 seeds were counted. Bar = 4 mm. *P < 0.05, Student’s t test. b Comparison of brown grain width between wild type HY and mutant cyp. Bar, 4 mm. Average brown grain width of HY and oscyp20–2 t1. At least 200 seeds were counted. c and d Statistics of brown grain thickness and 100-grain weight data of HY and oscyp20–2 t1, respectively. Error bar means SD. At least 200 seeds were measured. SD means six biological replicates in 100-grain weight statistics data. *P < 0.05, Student’s t test. e Cell morphology and average cell length of inner epidermal cells of lemmas in HY and oscyp20–2 t1. Scale bar, 20 μm. Error bar indicate SD for at least 30 cells. *P < 0.05, Student’s t test. f GUS staining of glume tissues of pOsCYP20–2:GUS transgenic plants at various stages of panicle from P2 to P6 stage (Jain et al. 2007) and qRT-PCR results of OsCYP20–2 expressing in different length of panicle. The panicles with indicated lengths were sampled for the analysis. g Panicle morphology of HY and oscyp20–2 t1 (cyp). The length of inflorescence and number of branches were compared between HY and oscyp20–2 t1. Scale bar, 2 cm. Error bar was calculated for at least 10 plants. *P < 0.05, Student’s t test