Table 4 Number of plants generated and recurrent parent genome recovery in the backcross generations during marker aided introgression of mutant AHAS allele in PB 1121
Generation | No. of plants generated | No. of plants carrying mutant AHAS allele in heterozygous/ homozygous state | No. of selected plants/families | Genome recovery (%) | Mean Observed RPG Recovery (%) | Expected Average Recovery (%) |
|---|---|---|---|---|---|---|
F1 | 15 | 15b | 1 | a | ||
BC1F1 | 262 | 128b | 1 | 70.98–80.35 | 75.66 | 75 |
BC2F1 | 188 | 94b | 1 | 81.25–92.85 | 87.05 | 87.5 |
BC3F1 | 152 | 73b | 1 | 93.30–95.08 | 94.19 | 93.8 |
BC4F1 | 122 | 58b | 1 | 94.64–98.21 | 96.42 | 96.9 |
BC4F2 | 384 | 88c | 40 | a | – | – |
BC4F3 | 40 | 40c | 12 | a | – | – |
BC4F4 | 12 | 12c | 12 | 98.66–99.55 | 99.10 | – |