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Fig. 7 | Rice

Fig. 7

From: UvAtg8-Mediated Autophagy Regulates Fungal Growth, Stress Responses, Conidiation, and Pathogenesis in Ustilaginoidea virens

Fig. 7

Deletion of UvATG8 resulted in decreased conidiation. a and b, Conidiation of the tested strains. The strains were cultured in 50 mL of PS medium with 170 rpm at 28 °C for 7 d prior to the observation. Three repetitions were performed, with similar results obtained. c, The expression of GFP-UvAtg8 during conidiation. d, Conidial germination of U. virens on rice sheath. 5 μL conidial droplets (1 × 106 conidia/mL) were inoculated and incubated at 28 °C for 3 d. Photographs were taken under an Olympus BX53 microscope equipped with bright field optics. e, The GFP-UvAtg8 was highly expressed, and autophagy had occurred during the secondary spore formation stage. The images were obtained using a confocal laser scanning microscope. Red arrow showed the conidial germination. Scale bar = 2.5 μm

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