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Fig. 6 | Rice

Fig. 6

From: UvAtg8-Mediated Autophagy Regulates Fungal Growth, Stress Responses, Conidiation, and Pathogenesis in Ustilaginoidea virens

Fig. 6

Virulence assays of the ∆Uvatg8 mutants. a, Disease symptoms caused by the WT, ∆Uvatg8, and ∆Uvatg8-C strains on 21 d after inoculation. b, Statistical analysis results of the average number of false smut balls per spikelet. At least three independent biological experiments were performed with 30 inoculated panicles each time. c, Culture filtrates of the ∆Uvatg8 mutants displayed less inhibitory of rice seedling germination. The shoot lengths were measured and statistically analyzed after 7 d in 28 °C illumination incubators. d, The growths of the rice shoots of the indicated strains were treated with testing culture filtrates. More than 100 rice seeds were sterilized with 0.1% potassium permanganate, then shifted to 35 mL culture filtrates at 28 °C for 7 d. e, qRT-PCR analysis of the expression of UvUSTA in the WT, ∆Uvatg8, and ∆Uvatg8-C strains. β-tubulin gene was used as the endogenous reference gene. Three independent experiments (n = 100) were carried out (mean ± SD). *** indicates p < 0.001

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