Fig. 4

Validation of the effect of qSD3.1 on seed dormancy. a Graphical genotype showing NIL (qSD3.1) that carries a single introduced Nipponbare segment encompassing qSD3.1. The red and green bars represent the Nipponbare segment and the 9311 background, respectively. b Germination curve of freshly harvested seeds of NIL (qSD3.1) and 9311. c Germination curve of NIL (qSD3.1) and 9311 seeds after dormancy breaks. d-g Germination behaviors of NIL (qSD3.1) and 9311; G_3d (d), AUC (e), T₅₀ (f) and G_7d (g). G_3d, germination rate at 72 h after imbibition; G_7d, maximum germination rate at 168 h after imbibition; T₅₀, germination speed, which is the time to reach 50% germination of seeds; and AUC, the area under the curve up to 168 h after imbibition. The error bar represents the mean ± SD (n = 3). Double asterisks indicate significant differences at P < 0.01