Fig. 3

Validation of the effect of qSD3.2 on seed dormancy. a Graphical genotype showing one CSSL (NY38) carrying an introduced Nipponbare segment encompassing qSD3.2 on chromosome 3 and a background Nipponbare segment on chromosome 10 in the 9311 background. b QTLs detected in the NY38-derived population (n = 105). LOD, logarithm of odds; Add, additive effect; Dom, dominance effect; PVE, phenotypic variance explained by the QTL. c Germination curve of freshly harvested seeds of NIL (qSD3.2) and 9311. d Germination curve of NIL (qSD3.2) and 9311 seeds after dormancy breaks. e-h Germination behaviors of NIL (qSD3.2) and 9311 seeds. G_3d (e), AUC (f), T₅₀ (g) and G_7d (h). G_3d, germination rate at 72 h after imbibition; G_7d, maximum germination rate at 168 h after imbibition; T₅₀, germination speed, which is the time to reach 50% germination of seeds; and AUC, the area under the curve until 168 h after imbibition. The error bar represents the mean ± SD (n = 3). Double asterisks denote significant differences at P < 0.01