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Fig. 3 | Rice

Fig. 3

From: Isolation of TSCD11 Gene for Early Chloroplast Development under High Temperature in Rice

Fig. 3

Map-based cloning of TSCD11 and transgenic complementation of the tscd11 mutant. a, b The TSCD11 locus was initially mapped on chromosome 11. c, Fine mapping the TSCD11 locus. d, TSCD11 gene structure and mutation site of TSCD11. e, phenotypes of wild-type, tscd11 with pCMBIA1300 (pCK/tscd11) and three independent tscd11 complementation plants (COM-1, 2 and 3) at 35 °C (bar = 2 cm). f, pigment contents of wild-type, tscd11 and complementation plants g, Relative transcript levels of TSCD11 in wild-type, tscd11 and complementation plants at the 3-leaf stage at 35 °C. The rice Histone gene was used as an internal control. The transcript level of TSCD11 in the WT at 35 °C was set to 1.0. Data represent the mean ± SD (n = 3). ** p < 0.01 (Student’s t-test)

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