Fig. 9

RNA-seq analysis of wild-type and wsl9 seedlings under 20 °C conditions. mRNA enriched from total RNA isolated from 10-d-old seedlings of WT and wsl9 mutant using oligo-(dT) was fragmented and reverse-transcribed using random hexamer primers. The library was then constructed and sequenced using an Illumina HiSEquation 2000. a Frequencies of detected genes sorted according to expression levels. b Read numbers of WT and wsl9 mutant sequences. c Volcano plot showing overall alterations in gene expression in WT and wsl9 mutant.d qRT-PCR of genes differentially expressed in RNA-seq. Five up-regulated and four down-regulated genes were assayed. Error bars represent SD from three independent experiments. (Student’s t-test, **, P < 0.01)