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Fig. 4 | Rice

Fig. 4

From: WSL9 Encodes an HNH Endonuclease Domain-Containing Protein that Is Essential for Early Chloroplast Development in Rice

Fig. 4

Map-based cloning of the WSL9 locus and identification of the mutation site in wsl9 mutant.aWSL9 was mapped to a 89 kb region flanked by N12 and N3–11 in chromosome 3; 16 open reading frames (ORFs) were predicted in the mapped region. b Structural model of the WSL9 gene. ATG and TGA represent the start and stop codons, respectively. Blue boxes indicate exons, lines between boxes indicate introns, and white boxes indicate the 5′- and 3′-UTR. One of the single nucleotide changes resulted in a missense mutation. c Verification of the difference between wild-type and wsl9 genomic DNAs using dCAPs markers. d Complementation of wsl9 by transformation. Scale bar, 2 cm

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