Fig. 7

OsDCL1 negatively regulates rice blast resistance. a and b Amounts of Osa-miR162a and mRNA levels of OsDCL1 in OX162 (a) and MIM162 lines (b) in comparison with Nipponbare (NPB). Total RNA was used to carry out reverse-transcription (RT) with an Osa-miR162 specific stem-loop RT primer (Additional file 4: Table S1), and the RT product was subsequently used as a template for quantitative polymerase chain reaction (q-PCR) to detect the amounts of Osa-miR162. snRNA U6 served as an internal reference. c Expression of OsDCL1 in susceptible accession LTH and resistance accessions (IRBLKm-Ts and Yahui2115) with or without M. oryzae strain Guy11 infection. RNA was extracted at the indicated time points for qRT-PCR analysis. The relative mRNA levels were normalized with the mRNA level of the mock sample at 0 hpi. d mRNA levels of OsDCL1 in OsDCL1 RNA inference transgenic line (DCL1i) and NPB plants. e Blast disease phenotypes on leaves of DCL1i and NPB at 5 days post-inoculation (dpi) of M. oryzae strain GZ8. Bar = 5 mm. f Quantification analysis of the fungal biomass in (e). Relative fungal biomass was measured by using the ratio of DNA level of M. oryzae Pot2 gene against the rice genomic ubiquitin DNA level. For a, b, c, d, and f, error bars indicate SD (n = 3). Different letters above the bars indicate a significant difference (P < 0.05) as determined by a one-way ANOVA analysis. All the experiments were repeated two times with similar results