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Fig. 5 | Rice

Fig. 5

From: Osa-miR162a fine-tunes rice resistance to Magnaporthe oryzae and Yield

Fig. 5

Blocking Osa-miR162a compromises the induction of disease-related defense responses. a Hydrogen peroxide (H2O2) accumulation in wild type (Nipponbare, NPB) and MIM162 at 48 h post-inoculation (hpi) of M. oryzae strain GZ8. The intensity of brown indicates the amounts of H2O2, which was stained by 3,3′-diaminobenzidine (DAB). The red arrows indicate appressoria formed from conidia. The upper photos were taken with a stereo-microscope, scale bars = 1 mm. The photos at the down portion were taken with a microscope (Zeiss imager A2), scale bars = 40 μm. b Expression of the defense-related genes (OsKS4, OsNAC4, OsPR1, and OsPR10b) in NPB and MIM162 following GZ8 inoculation. Rice ubiquitin (UBQ) gene was served as an internal reference. The mRNA levels were normalized to that in NPB at 0 hpi. Error bars indicate SD (n = 3). Different letters above the bars indicate significant differences (P < 0.05) as determined by One-way ANOVA analysis. Similar results were obtained in at least two independent experiments

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