Fig. 3
From: Osa-miR162a fine-tunes rice resistance to Magnaporthe oryzae and Yield
Osa-miR162a enhances the induction of disease-related defense responses. a Hydrogen peroxide (H2O2) accumulation in wild type (Nipponbare, NPB) and OX162 at 48 h post-inoculation (hpi) of M. oryzae strain GZ8. The intensity of brown indicates the amounts of H2O2, which was stained by 3,3′-diaminobenzidine (DAB). The red arrows indicate appressoria formed from conidia. The above photos were taken with a stereo-microscope, scale bars = 1 mm. The photos at the down portion were taken with a microscope (Zeiss imager A2), scale bars = 40 μm. b Relative mRNA levels of the defense-related genes (OsKS4, OsNAC4, OsPR1, and OsPR10b) in NPB and OX162 following inoculation of M. oryzae GZ8. Rice ubiquitin (UBQ) gene was served as an internal reference. The mRNA levels were normalized to that in NPB at 0 hpi. Error bars indicate SD (n = 3). Different letters above the bars indicate significant differences (P < 0.05) as determined by One-way ANOVA analysis. Similar results were obtained in at least two independent experiments