Agrobacterium-Mediated Genetic Transformation of Wild Oryza Species Using Immature Embryos

Shimizu-Sato, Sae; Tsuda, Katsutoshi; Nosaka-Takahashi, Misuzu; Suzuki, Toshiya; Ono, Seijiro; Ta, Kim Nhung; Yoshida, Yuri; Nonomura, Ken-Ichi; Sato, Yutaka

doi:10.1186/s12284-020-00394-4

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Table 5 Efficiency of Agrobacterium infection of calli induced from immature embryos of 76 accessions of wild Oryza

From: Agrobacterium-Mediated Genetic Transformation of Wild Oryza Species Using Immature Embryos

Species, genome type	Strain	LBA4404		EHA105		Infection efficiency^a
Species, genome type	Centrifugation	1100×g	20,000×g	1100×g	20,000×g	Infection efficiency^a
O. barthii, AA	W1467^b	1/9	2/8	0/8	1/8	3/6 (50%)
	W1473	0/8	0/8
	W1583				1/8
	W1588		0/8		0/8
	W1643		0/8		0/8
	W1702		2/9
O. glumaepatula, AA	W1183		1/8			13/14 (93%)
	W1189	0/8	0/8
	W1191		2/8
	W1477	1/8	3/16
	W2140	1/8	1/8
	W2145		1/8
	W2149	2/8
	W2160		2/8		3/16
	W2165	1/8
	W2173		3/16		2/16
	W2184^b	3/8	3/9	1/8	2/8
	W2192	2/8
	W2201	1/8	1/8
	W2203	1/8	2/8
O. longistaminata, AA	W1573^b	0/8	0/8	0/8	1/8	1/1 (100%)
O. meridionalis, AA	W1300	0/9	0/8			1/7 (14%)
	W1625		0/8	0/8
	W1631		0/8	0/8
	W1635	0/8	0/7
	W2071	0/8	0/8
	W2080^b	0/9	1/8	0/8	0/8
	W2105		0/8	0/8
O. rufipogon, AA	W0102		1/8			21/26 (81%)
	W0106	1/8
	W0107	2/8	2/16
	W0137	0/8	0/8
	W0593		1/8
	W0610		1/8		1/8
	W0621	1/8	1/8
	W0625	1/9	1/8
	W0629	1/8	1/8
	W1230	0/8	0/8
	W1239	2/8
	W1551^b	3/8	2/8	0/8	1/8
	W1666	1/8
	W1669	2/8
	W1681	1/8
	W1685	2/8	2/8
	W1718	0/8	0/8
	W1739	0/8	0/8
	W1852		1/8
	W1865	2/8	1/8
	W1945		1/8
	W1962		0/8		0/8
	W2014	2/8			1/8
	W2051		1/8
	W2263		1/8
	W2265	1/8	2/8
O. punctata (2x), BB	W1582^b	2/8	0/8	0/8	0/8	3/4 (75%)
	W1586	1/16
	W1592	0/8	0/8
	W1593	1/16
O. eichingeri, CC	W1525^b	0/8	0/8	1/8	0/8	1/1 (100%)
O. officinalis, CC	W1301^b	0/16	0/16	0/17	0/16	0/2 (0%)
O. officinalis, CC	W1830	0/8	0/8			0/2 (0%)
O. rhizomatis, CC	W1808^b	0/16	0/15	0/16	0/17	0/2 (0%)
O. rhizomatis, CC	W1812		0/8	0/8		0/2 (0%)
O. minuta, BBCC	W0051^b	0/8	0/8	1/8	0/8	2/3 (67%)
	W1323				1/8
	W1331			0/8	0/8
O. punctata (4x), BBCC	W1474(B)			1/8		2/2 (100%)
O. punctata (4x), BBCC	W1564^b	0/8	0/8	1/8	0/8	2/2 (100%)
O. alta, CCDD	W0017^b	1/8	1/8	0/8	0/8	1/1 (100%)
O. grandiglumis, CCDD	W2220^b	0/16	0/18	0/16	0/18	0/1 (0%)
O. latifolia, CCDD	W1177^b	0/24	0/25	0/24	0/24	0/1 (0%)
O. brachyantha, FF	W0656	2/8	2/8		2/8	3/4 (75%)
	W1407(B)	1/8
	W1706	0/8			0/8
	W1711^b	2/9	1/8	0/8	1/8
O. longiglumis, HHJJ	W1227^b	0/24	0/24	0/25	0/24	0/1 (0%)
Total						51/76 (67%)

number of GFP positive calli / total number of tested immature embryo
^a100 × number of GFP positive accessions / total number of tested accessions
^bsame data as Table 4

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