Skip to main content

Table 5 Efficiency of Agrobacterium infection of calli induced from immature embryos of 76 accessions of wild Oryza

From: Agrobacterium-Mediated Genetic Transformation of Wild Oryza Species Using Immature Embryos

Species, genome typeStrainLBA4404EHA105Infection efficiencya
Centrifugation1100×g20,000×g1100×g20,000×g
O. barthii, AAW1467b1/92/80/81/83/6 (50%)
W14730/80/8  
W1583   1/8
W1588 0/8 0/8
W1643 0/8 0/8
W1702 2/9  
O. glumaepatula, AAW1183 1/8  13/14 (93%)
W11890/80/8  
W1191 2/8  
W14771/83/16  
W21401/81/8  
W2145 1/8  
W21492/8   
W2160 2/8 3/16
W21651/8   
W2173 3/16 2/16
W2184b3/83/91/82/8
W21922/8   
W22011/81/8  
W22031/82/8  
O. longistaminata, AAW1573b0/80/80/81/81/1 (100%)
O. meridionalis, AAW13000/90/8  1/7 (14%)
W1625 0/80/8 
W1631 0/80/8 
W16350/80/7  
W20710/80/8  
W2080b0/91/80/80/8
W2105 0/80/8 
O. rufipogon, AAW0102 1/8  21/26 (81%)
W01061/8   
W01072/82/16  
W01370/80/8  
W0593 1/8  
W0610 1/8 1/8
W06211/81/8  
W06251/91/8  
W06291/81/8  
W12300/80/8  
W12392/8   
W1551b3/82/80/81/8
W16661/8   
W16692/8   
W16811/8   
W16852/82/8  
W17180/80/8  
W17390/80/8  
W1852 1/8  
W18652/81/8  
W1945 1/8  
W1962 0/8 0/8
W20142/8  1/8
W2051 1/8  
W2263 1/8  
W22651/82/8  
O. punctata (2x), BBW1582b2/80/80/80/83/4 (75%)
W15861/16   
W15920/80/8  
W15931/16   
O. eichingeri, CCW1525b0/80/81/80/81/1 (100%)
O. officinalis, CCW1301b0/160/160/170/160/2 (0%)
W18300/80/8  
O. rhizomatis, CCW1808b0/160/150/160/170/2 (0%)
W1812 0/80/8 
O. minuta, BBCCW0051b0/80/81/80/82/3 (67%)
W1323   1/8
W1331  0/80/8
O. punctata (4x), BBCCW1474(B)  1/8 2/2 (100%)
W1564b0/80/81/80/8
O. alta, CCDDW0017b1/81/80/80/81/1 (100%)
O. grandiglumis, CCDDW2220b0/160/180/160/180/1 (0%)
O. latifolia, CCDDW1177b0/240/250/240/240/1 (0%)
O. brachyantha, FFW06562/82/8 2/83/4 (75%)
W1407(B)1/8   
W17060/8  0/8
W1711b2/91/80/81/8
O. longiglumis, HHJJW1227b0/240/240/250/240/1 (0%)
Total     51/76 (67%)
  1. number of GFP positive calli / total number of tested immature embryo
  2. a100 × number of GFP positive accessions / total number of tested accessions
  3. bsame data as Table 4