Table 3 Summary of regeneration tests from immature embryos of wild Oryza species
From: Agrobacterium-Mediated Genetic Transformation of Wild Oryza Species Using Immature Embryos
Species, genome type | No. accessions tested | No. accessions with callus inductiona | No. accessions with regenerationb | Percentage of regenerated accessionsc |
|---|---|---|---|---|
O. barthii, AA | 10 | 8 (80%) | 7 (88%) | 70% |
O. glumaepatula, AA | 18 | 17 (94%) | 14 (82%) | 78% |
O. longistaminata, AA | 1 | 1 (100%) | 1 (100%) | 100% |
O. meridionalis, AA | 15 | 13 (87%) | 7 (54%) | 47% |
O. rufipogon, AA | 44 | 39 (89%) | 28 (72%) | 64% |
O. punctata (2x), BB | 8 | 8 (100%) | 5 (63%) | 63% |
O. eichingeri, CC | 2 | 1 (50%) | 1 (100%) | 50% |
O. officinalis, CC | 16 | 7 (44%) | 4 (57%) | 25% |
O. rhizomatis, CC | 3 | 3 (100%) | 2 (67%) | 67% |
O. minuta, BBCC | 12 | 10 (83%) | 5 (50%) | 42% |
O. punctata (4x), BBCC | 8 | 4 (50%) | 2 (50%) | 25% |
O. alta, CCDD | 3 | 2 (67%) | 1 (50%) | 33% |
O. grandiglumis, CCDD | 7 | 6 (86%) | 6 (100%) | 86% |
O. latifolia, CCDD | 10 | 4 (40%) | 2 (50%) | 20% |
O. australiensis, EE | 10 | 5 (50%) | 0 (0%) | 0% |
O. brachyantha, FF | 7 | 6 (86%) | 4 (67%) | 57% |
O. granulata, GG | 4 | 2 (50%) | 0 (0%) | 0% |
O. meyeriana, GG | 2 | 1 (50%) | 0 (0%) | 0% |
O. longiglumis, HHJJ | 9 | 1 (11%) | 1 (100%) | 11% |
O. ridleyi, HHJJ | 3 | 0 (0%) | 0 (0%) | 0% |
Total | 192 | 138 (72%) | 90 (65%) | 47% |