Fig. 3
From: Agrobacterium-Mediated Genetic Transformation of Wild Oryza Species Using Immature Embryos
Detection of transgenes in four lines of T0 plants from 4 accessions. a, eO. barthii W1467, b, fO. glumaepatula W2184, c, gO. rufipogon W1551, and d, hO. brachyantha W1711. a–d PCR amplification of full-length hpt gene from genomic DNA prepared from each independent line of transgenic plants (T0). Lanes: M, molecular weight marker; W, non-transgenic control; L1–L4: 4 independent lines of each transgenic wild Oryza accession. e–h Southern blot analysis of transgenic T0 plants with hpt probe. Lanes: W, non-transgenic control; L1–L4, T0 plants of the same lines in (a–d)