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Fig. 3 | Rice

Fig. 3

From: Agrobacterium-Mediated Genetic Transformation of Wild Oryza Species Using Immature Embryos

Fig. 3

Detection of transgenes in four lines of T0 plants from 4 accessions. a, eO. barthii W1467, b, fO. glumaepatula W2184, c, gO. rufipogon W1551, and d, hO. brachyantha W1711. ad PCR amplification of full-length hpt gene from genomic DNA prepared from each independent line of transgenic plants (T0). Lanes: M, molecular weight marker; W, non-transgenic control; L1–L4: 4 independent lines of each transgenic wild Oryza accession. eh Southern blot analysis of transgenic T0 plants with hpt probe. Lanes: W, non-transgenic control; L1–L4, T0 plants of the same lines in (ad)

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